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Behavioral endpoints and generational effects in Daphnia magna upon short- and long-term exposure and their use as additional endpoint in ecotoxicological risk assessment

Daphnia magna is a common model organism for ecotoxicological testing. In regulatory guidelines, the classical tested endpoints are immobilization and reproduction. In order to detect other sublethal effects potentially more sensitive endpoints are required. Behavioral endpoints are one approach in environmental risk assessment for more sensitive endpoints and monitoring of water quality, demonstrating ecological relevance since altered behavior can impact reproduction and survival, yet they are rarely included in risk assessment. In literature, several chemicals have shown to alter swimming behavior of D. magna. The objective of our study was to identify suitable behavioral endpoints regarding sensitivity and applicability under short-term and long-term exposure to selected insecticides in environmentally relevant concentrations and potential generational effects of insecticide exposure. The investigated …

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