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Behavioural phenotyping with small aquatic model organisms: why digital video resolution and bitrate matter

Behavioural analysis with small aquatic model organisms is increasingly applied across diverse areas of biosciences and biomedicine, including ecology, pharmacology, and experimental neurobiology. High-throughput behavioural phenotyping workflows frequently employ organisms such as larval zebrafish ( Danio rerio ) in multiwell laboratory plates, yet the considerable cost of commercial imaging platforms often limits access for smaller laboratories, early career investigators and researchers in low- and middle-income countries. Here, we describe a rapid and practical approach for constructing an inexpensive, flexible and fully customisable behavioural data acquisition system suitable for use with a broad range of aquatic model species. We also examine an important but frequently overlooked methodological issue in quantitative behavioural research: how video acquisition parameters, particularly resolution and bitrate, influence …

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