Volta

Growth dynamics of 3,909 Escherichia coli single-gene knockouts in rich and minimal media

High-throughput phenotyping of microbial growth is crucial for understanding genotype–phenotype relationships in systems biology. Linking genetic variation to dynamic growth responses across environments remains challenging. Here, we present a time series dataset representing the growth curves of 3,909 single-gene knockout Escherichia coli strains grown in rich (LB) and minimal (M63) media. Using microplate assays with biological triplicates at 37 °C, we generated 23,454 OD 600 time-series trajectories (3,909 strains × 2 media × 3 replicates) recorded every 15 minutes for 24–48 hours. The dataset provides plate-background–corrected growth curves, derived growth parameters including carrying capacity ( K ) and maximal growth rate ( r ), and gene category annotations. This standardized resource facilitates comparative analyses of genotype-dependent growth dynamics between rich …

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