Volta

Operationalizing SARS-CoV-2 wastewater monitoring to assess traveler health in Las Vegas, Nevada, USA

Objectives: This study explored expanded traveler- and tourism-focused wastewater monitoring in Las Vegas, Nevada, USA to complement community SARS-CoV-2 surveillance. Methods: Wastewater samples were collected November 2023 to July 2024 from the largest community-scale wastewater treatment plant in Southern Nevada, USA (N = 112 samples) and two upstream utility access holes (i.e. manholes), isolating an international airport (N = 68 samples) and a commercial area with high-density bars and nightclubs (N = 30-33 samples). Polymerase chain reaction-based methods quantified RNA concentrations of SARS-CoV-2 and pepper mild mottle virus; whole genome sequencing characterized SARS-CoV-2 variants (N = 83 qualifying samples). Results: SARS-CoV-2 concentrations exhibited concordance between liquids- and solids-based approaches. Similar trends were observed between methods and sampling locations; however, select …

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