Volta

Exploring the impact of PVC and PVA microplastics on zebrafish tissue using multi-spectral imaging, Optical Coherence Tomography (OCT) and biospeckle OCT (bOCT)

Plastics are widely used in industry and households, but improper disposal has caused their accumulation in aquatic systems worldwide. As a result, mechanical and photochemical processes break down these plastics into microplastics or nano plastics, posing a severe threat to marine organisms and humans as they enter the food chain. This study investigates the effect of Polyvinyl chloride (PVC) and Polyvinyl alcohol (PVA) microplastics in zebrafish by using multi-spectral imaging (MSI), Optical Coherence Tomography (OCT), and Biospeckle OCT (bOCT). These techniques allow for long-term studies in the fish without invasive procedures in real-time. Zebrafish were exposed to Nile red labeled PVC and PVA for 21 days with 500mg/L concentration. Image acquisition and analysis were performed every five days till the …

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