Volta

Aircraft Wastewater as a Lens On Global Pathogen Spread: Matrix-Induced Challenges and Workflow Optimization for SARS-CoV-2 RNA Detection and Sequencing

High Resolution Image Download MS PowerPoint Slide Aircraft wastewater (AWW) surveillance emerged as a promising tool to monitor transboundary pathogen introduction during the COVID-19 pandemic. Reliable viral RNA detection and sequencing are essential for wastewater-based epidemiology, yet systematic evaluation of AWW processing methods remains limited. This study assessed the impact of disinfectant on SARS-CoV-2 RNA recovery and developed an optimized workflow to maximize RNA yield and quality for PCR-based quantification and sequencing. Even low concentrations of disinfectant (Aeroclean-T-600) caused substantial RNA loss in municipal wastewater (e.g., half-life of 1.1 h at 0.25%), underscoring the need for cautious data interpretation. However, high SARS-CoV-2 RNA positivity rates in this study (72%) and elsewhere suggest that sample matrix effects and different disinfectant chemistry …

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